Endometrial dating infertility
CONCLUSIONS: The correlation found between the endometrial datings suggests that biopsies performed on either of these two days are suitable for evaluation of endometrial maturation. All procedures were also approved by the Medical Ethics Committee of the Universidade Federal de São Paulo.
Blood samples were drawn from patients between days one and five of the menstrual cycle, for basal plasma levels of LH, FSH and prolactin, measured by immunofluorimetry (normal ranges: FSH: 2.4 to 9.3 U/L; LH: 1.5 to 10 U/L; prolactin up to 14.5 ng/ml).
Endometrial maturity was evaluated through morphometric analysis, taking into account four semi-quantitative parameters (amount of luminal secretion in the gland, pseudo-stratification of the glandular epithelium, stromal differentiation and edema), estimated on a scale from 0 to 3, and two quantitative parameters (number of supra- and subnuclear vacuoles in the glandular epithelium and the volume fraction of the gland occupied by the epithelium). According to the morphometric criteria, the endometrium was considered as immature (out of phase) if histological analysis showed a maturation delay of one or more days.
In the first of these, the catheter was introduced with its subterminal orifice directed to the right uterine wall and in the second, to the left.No entanto, não existe um consenso em relação ao período da fase lútea mais adequado para a colheita.OBJETIVO: Avaliar a correlação entre as datações histológicas de duas amostras de endométrio colhidas nos dias 6 e 10 da fase lútea de um mesmo ciclo menstrual.Mean endometrial thickness on day LH 0 was 10.3 mm (range 7 to 13 mm; standard deviation 1.4 mm).All patients showed a normal uterine cavity on hysterosalpingogram and histological analysis showed no endometrial pathology.
On day LH 6, blood was drawn for plasma progesterone level determination.